Nitrite reductase from Achromobacter fischeri
نویسندگان
چکیده
منابع مشابه
Nitrite reductase from Achromobacter xylosoxidans forms stable trimers in dilute solution.
Nitrite reductase is a key metabolic enzyme in denitrifying anaerobic bacteria. In the past, nitrite reductase purified from a number of microorganisms has shown variation in subunit composition. In this study we show definitively using Sedimentation equilibrium i n the analytical ultracentrifuge that nitrite reductase from the species Achromobacter xylosoxiduns exists as stable trimers i n dil...
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Microbial nitrite reductases are denitrifying enzymes that are a major component of the global nitrogen cycle. Multiple structures measured from one crystal (MSOX data) of copper nitrite reductase at 240 K, together with molecular-dynamics simulations, have revealed protein dynamics at the type 2 copper site that are significant for its catalytic properties and for the entry and exit of solvent...
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Resonance Raman spectroscopy at ambient temperature and 77 K has been used to probe the structures of the copper sites in Achromobacter cycloclastes nitrite reductase. This enzyme contains three copper ions per protein molecule and has two principal electronic absorption bands with lambda max values of 458 and 585 nm. Comparisons between the resonance Raman spectra of nitrite reductase and blue...
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Nitrite reductase from Escherichia coli K12 requires the presence of NAD+, one of the products of the reduction ofNO2by NADH, for full activity. The effect is observed with both crude extracts and purified enzyme. NAD+ also acts as a product inhibitor at high concentrations, and plots of initial rate against NAD+ concentration are bell-shaped. The maximum occurs at about 1 mM-NAD+, but increase...
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1. In rice seedlings synthesis of methyl viologen-nitrite reductase was stimulated by light, as was that of NADH-nitrate oxidoreductase (EC 1.6.6.1). A small residual effect of light on the synthesis of the enzymes persisted in the dark for a short time. 2. In etiolated seedlings exposed to light and nitrate, a lag period of 3 h was necessary before enzyme synthesis commenced, whereas in green ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1981
ISSN: 0378-1097
DOI: 10.1016/0378-1097(81)90230-5